FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-A12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-A12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065391
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG2551.1241355E-917.848571
CCCCTGA2601.4570105E-917.505331
TCCTAAG5050.016.6351032
TAGGCCG1503.1597482E-416.3339545
CCCCTAG2009.942487E-615.7547961
CGCGCGG1807.158857E-515.55464154
GTCCTAT4450.014.948371
TACACGG2403.4497825E-614.5838885
GTATTAG8550.014.3318471
CCTTGCG6600.014.318389
CTTACAC5300.013.8684523
CCGGCCG1800.001260195413.61064164
CCTATAC7250.013.51775553
CCTACAC3901.54796E-913.4620513
GCTATAC6000.013.4171773
ATCCTAG3951.8662831E-913.2951861
GCGAGTC1850.001548224413.24406846
TCCTTGC12700.013.228538
GTATATA21450.013.2208071
ACGGGCC2659.623112E-613.20677121
Produced by FastQC (version 0.11.5)