FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-A11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-A11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181711
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTGC12750.015.9231748
GTCCTAC3351.5643309E-1015.6782541
GTATCCT9000.015.56211951
TCCTATA9000.015.5585512
GTCCTAA4750.015.4802111
CCTTGCG6650.015.2646979
GTATTAG10900.014.7768741
TCCGCAC3655.7480065E-1014.38171564
CCTATAC7750.013.9994873
CGGCTGG2607.9125075E-613.4598115
CCTCTTA6000.013.4223271
GCCTCCT4052.7048372E-912.9684321
CGCACCT3254.247504E-712.92141866
CGGGTCG1900.001895158812.89308218
GCGAACC3002.4434248E-612.83168623
GTCCCCT2751.4022877E-512.7326421
TCCTAGG2508.146773E-512.6024262
CTATGCA8400.012.5012629
TAGTACA8200.012.3792974
CTAGACT5155.0931703E-1112.2342444
Produced by FastQC (version 0.11.5)