FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP2-F12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP2-F12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655126
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT2456.6211214E-1018.5722894
CGCGCAC1150.001007960518.26171310
GCGCACT2201.3968274E-615.90790311
TCTATAC8250.015.2725093
TAGTCAG3604.656613E-1014.5840079
CCCTAGG1450.004725373414.4899931
TAGGTAG3901.0186341E-1014.3596385
TATACAG11300.014.24844555
GTATATA18000.014.0069931
GTATTAG6800.013.9040011
ACGGTGT5550.013.8745158
TATACGG6200.013.5490135
GGTAGTC4002.2591848E-913.1256067
TATACTG9750.012.9236745
TACACTG6500.012.9236745
GTATAGA7650.012.8168581
CATATAC11850.012.7002413
GTCCTAC2508.121112E-512.6062961
CGGTGTA2254.7489424E-412.4450199
CTTACAC4509.804353E-1012.4442673
Produced by FastQC (version 0.11.5)