FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP2-E07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP2-E07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964781
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT3550.021.6878366
TCCGCAC3650.021.09364564
CCGCACC3950.019.49159465
GTATAGA9200.016.748431
CTAATAC8650.016.1844843
CCTAATA9900.014.4978332
AGGCGTC3403.265086E-914.4102320
TAGGACC3655.7298166E-1014.3849594
GTCTAAG4403.6379788E-1214.3261371
TACACCG3951.2369128E-1014.1785925
ACTATGC8700.014.081118
GGCGTCC2759.180403E-713.99850821
TACTATG9450.013.7043367
TAGGTAG4253.965397E-1013.177755
AGACTGG5051.8189894E-1213.1689256
GTATTAG9900.013.0880761
GGCGTCG2952.0192601E-613.05145132
ACGGGCT2952.0192601E-613.05145133
AGCGGGG2456.764469E-512.85511950
GTATAGG6000.012.8404621
Produced by FastQC (version 0.11.5)