FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP2-E06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP2-E06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222243
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAC2603.6379788E-1220.1912213
ACAGTGC2453.092282E-1120.0030168
GCGACCG900.005277444619.44419369
ACCACGC1200.00133977917.502648
AGCGGGG1250.001764279816.79772250
TCCGCAC1755.6278503E-515.99782964
CTATACT6000.015.7523764
TAGGCTG1350.002943785915.5579035
TACACCG2251.7603252E-615.5579025
AGGACCG1400.003750338215.0022625
CGCACCT2101.583744E-514.99796666
CTGGCCC1656.513093E-414.8507259
GTATATA12050.014.5285791
GGACCGT1450.004735385514.4849426
TAGGTAG2707.4350646E-714.2614115
GAGTCCG1500.00592958714.0021119
TAGACAG3001.595472E-714.0021115
CAGATCA3555.9644663E-913.8048999
GTATTAG5350.013.7437631
GTGTTAG4353.8198777E-1113.6835841
Produced by FastQC (version 0.11.5)