FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP2-E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP2-E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705336
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC1101.0170988E-625.45537838
GCCCCGA1854.749674E-617.0280848
CGCGCAT1807.144935E-515.55788840
CCTAGCC1605.1661115E-415.3116533
CCCTAAG2352.7601855E-614.900221
CTAGGAC1901.1349579E-414.7360283
CCCGATT2505.2635132E-614.00086810
TCCTAAG3303.4197E-813.7875212
GTATAGG5100.013.7315761
CCCCGAT2556.4598116E-613.7263419
CCTCGGA2303.730072E-513.6965029
GTATTAG6650.013.6902781
CTCCTAG2052.1552E-413.6645931
TGACGCG2052.162715E-413.65898337
CCCTTGC4404.7293724E-1113.5235658
TTACCTC6300.013.3325973
CTTACAC3456.125447E-813.1876763
CGGGCGG2405.5709843E-513.1238952
CCTACAC2701.1663642E-512.9622473
ATGCTAG6250.012.885711
Produced by FastQC (version 0.11.5)