FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP2-D10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP2-D10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495950
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAG500.00720937428.00133739
CCGGCGA952.7823081E-422.10484170
ACGGGCC850.003793913420.5857847
GGACCGC950.00720960618.4219346
GCGTGCG1000.00969836217.4979111
TAGACAG4250.015.6488535
TATACAG10250.015.3676155
GTCTAGA3503.0377123E-1015.0067361
GTATTAG7050.014.9003061
CACTGCG1656.519397E-414.8491947
TCCGCAC2604.869835E-714.80592564
GGCTAGC2151.9784582E-514.64941552
CTGGGCA2403.4478962E-614.584039
GTATATA21900.014.5499111
CCGGAGC1450.00474454914.481029513
GTATAGA8000.014.4439821
TATACTG11200.014.3766475
GGCGTCG2202.4547657E-514.31838632
CGCACCT2951.3043245E-714.23558966
GTCCTAG1500.005918714.0062871
Produced by FastQC (version 0.11.5)