FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP2-D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP2-D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2857494
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC1807.14905E-515.5575296
CATATAC19700.015.1010583
TATACGG12900.014.6532765
GTATAGA14400.014.589781
GTATTAG12800.014.2250351
TAGGTAG5700.014.1248455
CGACGGG2505.277292E-613.998149
GTATATA30400.013.9370781
TATTAGG12950.013.785292
TATACAG17200.013.6356875
GTATATG17100.013.3099741
TATATAC35500.013.2108623
ACTGCCC4002.2591848E-913.1266648
ACCGCGC2153.2350025E-413.0249068
ATACGGT12450.012.9333676
CGCTCGA2456.7485715E-512.85877210
ACGGTGT9750.012.5656968
ACCGTGC3103.5187059E-612.420938
TCATATA25050.012.2967532
ATACCGT10000.012.2515536
Produced by FastQC (version 0.11.5)