FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP1-F01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP1-F01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262547
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG10050.014.6282975
GTATTAG13300.014.2160591
TATACTG21100.014.1009065
GTATATA38550.014.0781121
TCCGCAC5750.013.38974264
GTATAGG10750.013.35403251
TCTAGAC7100.013.3091233
TAGATAG13450.013.2726755
CGCACCT6350.013.22680366
TATACGG11950.012.888295
TATACAG20200.012.8230375
GTACTAG8950.012.5188381
GTATAGA14850.012.4964451
CTAATAC15550.012.1536673
ACTATGC13850.012.1309658
CCGCACC6400.012.02984665
TTATACT23200.011.9194534
CTAGACT6550.011.7570564
ACACCGT9050.011.60316856
TATAGGG8450.011.5982352
Produced by FastQC (version 0.11.5)