FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP1-E04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP1-E04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3905620
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14950.015.4602121
CCGCACC6850.014.81580865
CTAATAC17100.014.7366513
TAGGTAG9400.014.1496575
TCCGCAC7500.013.998383564
GTATAGG13150.013.3154831
CGCACCT7700.013.18029766
GTATATA37000.012.6828191
TATACAG21750.012.0695825
GTATAGA17550.011.9725551
TATAGGG11450.011.6178012
GTATAAA27900.011.1711651
ACTATGC18300.011.0934628
GTATTAA22200.011.0422551
GACCGCG3853.2750977E-610.9096487
GTGTATA15750.010.8950251
TCTACAC14500.010.8619283
TAATACT30300.010.858644
ACAGTGC8850.010.6785118
GACCACG5956.4028427E-1010.5887767
Produced by FastQC (version 0.11.5)