FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP1-C04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP1-C04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3862820
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT6500.014.53697866
TATACAG22500.014.3118735
CTAATAC16300.014.1716163
TAGGTAG8400.014.167425
GTATAGA18050.013.7739311
CATATAC23200.013.72829153
GTATTAG15100.013.682071
TCCGCAC6950.013.59573464
CCGCACC6750.013.48010565
TATACGG13300.012.8954245
ACGGTGT10250.012.6346558
GTATATA37700.012.6320641
TAGTACT16500.012.5159814
TATACTG22450.012.4728245
TACGGTG11050.012.3534397
CTAGGAC5950.012.3527993
TAATACT29450.012.2418974
GTCTTAG9800.012.1486951
TAGACGG6100.012.0498235
CGACGGG3802.1636697E-711.97230749
Produced by FastQC (version 0.11.5)