FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP1-B07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP1-B07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4525708
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG18350.013.7409451
GTCCTAA9550.013.5680841
TATACCG13050.012.8750625
CCTATAC14050.012.4564143
GTATAGA19700.012.0882341
CTCCTAC14550.012.0344771
CTATACA21000.012.0013954
ATACCGT18050.011.8289786
TCCGCAC8000.011.81139564
CGCGCGG3903.0307092E-711.66557554
CTAGACT7550.011.5907524
TATACAG26100.011.533915
TATACTG26400.011.40284255
CGCGGGA4054.939993E-711.23351756
CTAATAC21250.011.2008093
GTATATA44850.011.1659011
GCTCCGC4451.3950194E-711.01288128
CCGACCC3501.3607554E-511.0006729
CTATACT19100.010.9960434
TAGGTAG9600.010.9387735
Produced by FastQC (version 0.11.5)