FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP1-A09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP1-A09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441314
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGGG604.976782E-429.1692626
CCCCTAG500.0071905528.0120161
GCGTAAC500.00721047227.9961547
CCTCTTA1207.357448E-826.2612651
CCTAATA2100.025.0022282
GTCCTAA851.3034315E-424.7164861
CTATGCA2051.8189894E-1223.9045689
TCCGCAC1652.6539055E-923.33012464
CGCGGGA750.001832716523.33012256
CGCGCGG750.001832716523.33012254
ACTATGC2056.002665E-1122.1970988
CCCCTCA800.002663191221.8769477
CAGCTCG800.002663191221.87694738
TAATACT2750.021.638294
ACCTAAT2301.2732926E-1121.3134921
CTAATAC2301.2732926E-1121.3014183
TGAATCC1356.006181E-620.7425864
TATACCG1356.006181E-620.7425865
GGGCTCG850.00378516520.59006910
AACCCTC850.00378516520.5900696
Produced by FastQC (version 0.11.5)