FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11296202
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG730430.6466155615843272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT9550.014.33346666
TAGGTAG9800.013.5496225
GTATTAG21150.013.4275661
CCGCACC9950.013.40264865
TATACAG30250.013.0533725
GTATAGA23050.012.7770611
TCCGCAC10850.012.28986964
CCTAGCG7150.012.25098530
GTATATA53850.012.1752681
CTAGCAC10550.011.9245723
ACTATGC20500.011.76489458
CTAATAC26550.011.7143513
CTAGACT9250.011.7115054
ACTGCGC6900.011.6512248
TAATACT41200.011.6202674
TATACGG16550.011.6127315
GTATAGG16750.011.5125241
TCTATAC22150.011.201533
CTCTTAG11950.011.1490621
TAAGAGT16950.010.9269494
Produced by FastQC (version 0.11.5)