FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_bc_anc5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_bc_anc5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647043
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGGG1506.3910556E-720.99923547
CCGCACC3250.019.3880365
TATACAG14150.019.0452425
CCTAGCG1702.1198048E-618.53042430
TCCGCAC3600.018.47547364
GTATAGA9900.018.0296461
CGCACCT3350.017.76432266
TCTATAC10250.016.3896473
GTATATA25750.016.174171
CGCGGGT1554.0576005E-415.80587637
GCGGGAG1554.0576005E-415.80587649
TAGGTAG3602.7284841E-1115.5549895
TATACGG6700.015.1487025
AGGTCCG2101.5829226E-514.999454536
ATACAGC9000.014.777246
TGGCGAC4550.014.61485250
AGCGTGT3604.656613E-1014.58368810
ACTATGC10100.014.5548098
TACTATG10650.014.4604457
GTATTAG8800.014.3176611
Produced by FastQC (version 0.11.5)