FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_bc_anc4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_bc_anc4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032006
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC4250.019.76674865
TCCGCAC4300.019.53690364
TAGGCCG2456.6211214E-1018.57106244
CGCACCT4850.018.043166
CTAGGGG1200.001341725417.4996573
GCGACCG1250.001763455116.7996737
GGCGTCG2954.3655746E-1016.6106632
CTAATAC9700.016.597613
CTAGGAC2602.7685019E-816.1535283
GGCTAGC3401.9645086E-1015.44087252
GTATAGA11850.015.3583471
ATCAGCC6600.014.84819254
GTGCGTG6000.014.58376332
TAGGACG2202.456409E-514.3178994
GTGGCGT3758.60382E-1014.00041330
CGCGCAC1500.00593711414.00006810
ACTATGC11850.013.8819258
CCTAATA12650.013.833722
GGCGTCC3607.2886905E-913.61084221
CTATGCA9550.013.5602769
Produced by FastQC (version 0.11.5)