FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_bc_anc3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_bc_anc3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885921
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTGCG8300.019.8192759
GTCCGGC2451.3293175E-817.14434233
TCCGCAC5200.016.82954664
GTATTAG10850.015.4833351
CCTGGAC4400.015.1131123
CGTCCGG2806.876144E-815.001332
TCCTTGC15950.014.9216318
TAGGTAG6900.014.7096355
CGCACCT5800.014.48501866
CCGCACC5800.014.48501865
ACCCGCG3151.85737E-814.44394534
TATACCG9450.014.4439445
CCCTTGC8800.014.3181838
TCCTAGC5650.014.2477878
CCGTCCG2951.3024692E-714.23852231
CCTATAC9650.014.14458753
CCCGCCG3758.62201E-1013.99951524
GTATAGA12000.013.9995141
CTCCTAG3801.0513759E-913.8153111
GTATCCT10350.013.5261011
Produced by FastQC (version 0.11.5)