FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_bc_anc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_bc_anc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1656542
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGC900.0052787519.44385711
CCGCACC2551.1259544E-917.8463765
CGCACCT2207.263043E-817.5031766
TAGGTAG3401.9645086E-1015.440715
ACTATGC6150.014.7963018
CGCGCAT1901.1346846E-414.73639740
ACCGCTC2656.035698E-714.5278638
TCCGCAC3001.5960359E-714.00253664
CTATGCC4001.5097612E-1013.99957759
CCTAGCC1500.00593788313.99957753
GTATAGA6950.013.5967111
GTAGCGT2851.3712524E-613.5083656
TATACCG4951.8189894E-1213.4339395
CCCTAGA2354.5695168E-513.4038512
GACAGCG2901.6674658E-613.2754627
ACGCGCA1850.001549077613.24284339
TATACGG5850.013.1619955
CTAATAC7500.013.0662733
CTAGGAC2508.1614635E-512.599623
ATAGCGC3407.327435E-712.3525698
Produced by FastQC (version 0.11.5)