FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_bc_anc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_bc_anc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450948
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC2704.4035914E-815.55574510
CGCACCT4403.6379788E-1214.32010966
TATATAC22400.014.0620993
TATACGG7550.013.4433255
GTATATA21100.012.938021
GTATTAG11650.012.9180861
TACTCCG3558.902134E-812.8165365
CCGCACC5255.456968E-1212.66837265
CCTAATA11950.012.5937822
GTATAGA10650.012.4879061
CACTGCG3651.278313E-712.4659057
CATATAC14700.012.142513
CCGTCCT2601.17728356E-412.1155339
TCCGCAC5601.8189894E-1111.87659964
ACTGCGC3551.2347027E-611.8311318
TAAGACT9000.011.6663334
TACAGCG5752.910383E-1111.5653597
TAATACT17700.011.4685994
ACTATGC10250.011.2684318
GACTAAG10300.011.21327269
Produced by FastQC (version 0.11.5)