FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_Gbc_c84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_Gbc_c84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741187
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGA1550.031.61172556
CGCGCGG1750.027.99895554
GTCCTAC2004.3655746E-1122.749151
AACGCGC2500.022.39916452
ACGCGCG2301.2732926E-1121.30355353
GCGCGGG2701.2369128E-1018.1474755
TCCTTGC9500.017.6845658
CGGGAAC2802.0736479E-1017.49934658
CGGGTCG1503.161206E-416.33272418
CCTACAC3950.015.9487713
TAGGTAG3552.1827873E-1115.7740585
CGTCCGG2051.2592029E-515.36660332
CCTTGCG4900.015.0003019
CCTACGC1400.00375588814.999443
GACGCGC2101.5830397E-514.99943938
GTATCCT6650.014.7362921
CTATACT9100.014.6148394
TATACTG9800.014.2851825
ACGCGCA2253.0359188E-513.99947739
TCCTACG2607.902887E-613.4610362
Produced by FastQC (version 0.11.5)