FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_Gbc_c82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_Gbc_c82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229168
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCCG252.3877574E-455.99633422
CACGGGC350.001254069939.99738320
GGCGCGA350.001254069939.99738338
AACCCTC501.7068778E-434.997716
CGTCGGC400.002416182334.99770734
GCGCACC450.00430271431.10907635
ACGGGCC450.00430271431.10907621
CGCACCG450.00430271431.10907636
GTCTTAA450.00430271431.1090761
CCTTTAG952.8095565E-729.4717541
CCTCTCA604.9739034E-429.1647571
CCGCCCG500.007193146728.00427827
ACCGGTC500.007200816627.99816735
CCGTCAC700.001224026324.99836326
ATGCTAC851.3051364E-424.7042667
GCTATAC750.001829273323.3318043
ATACCGT952.7732315E-422.1038156
GTATAGT800.002661709421.8735681
CAGTCTT800.002661709421.87356817
TACTATA1154.1031013E-521.3029542
Produced by FastQC (version 0.11.5)