FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_Gbc_c81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_Gbc_c81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889341
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG2603.6379788E-1220.19178254
GTATTAG11250.017.7328721
CGCGGGA3104.9112714E-1116.93504356
CTAATAC13050.015.5551513
ACTATGC14000.015.2501318
TCCGCAC5600.015.00220864
GCGCGGG3801.0513759E-913.81543155
AACGCGC3851.2787496E-913.6360152
GTATAGA12500.013.4396521
CCTTGCG7650.013.2680889
TATTAGG11750.013.1060422
CTATGCA10950.013.1051369
ACGCGCG4103.272362E-912.80454653
CCTAATA13150.012.7753342
TATACGG8850.012.6550385
CGCACCT6200.012.42118266
GTATCCT9950.012.3112371
TAGGTAG6300.012.2219055
TCCTTGC14950.012.1740198
GTATTAC12900.011.93767451
Produced by FastQC (version 0.11.5)