FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_Gbc_c80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_Gbc_c80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253408
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGG501.7069286E-434.9993140
ACTGGTC755.5718938E-527.9994498
ACTGCGC500.00720062727.9994478
TAGGTGC500.00720062727.99944726
GAGCTAT700.001224057724.9995066
GACTGGT1001.3933883E-524.4995167
GTGATAG750.001829318423.3328741
TCTTATC800.002661773221.8745698
GCTATGT800.002661773221.8745698
CCCTTAG800.002661773221.8745691
TATAGAG800.002661773221.8745692
GTATATA1003.9224938E-420.9995841
CCCTGAA850.00378316720.587832
AGACCAT850.00378316720.587835
CCGTTCA850.00378316720.587839
CTTACTA850.00378316720.587832
GGCATGA3100.020.3221870
TAATAGT1055.451152E-419.9996054
AGGCATG3500.019.99960569
ATCTTAT1055.451152E-419.9996051
Produced by FastQC (version 0.11.5)