FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_Gbc_c79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_Gbc_c79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40428
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATGC150.002209356370.01
ATATACA200.00690382652.5000044
TATTCAT200.00690382652.5000045
GGAGTGA200.00690382652.50000447
CCGCGCG200.00690382652.5000049
GTTCTTA350.00124144540.01
GACGGAC900.005190970419.44444570
CATCTCC6050.004685566.363636570
ACACATC7250.00468738055.793103770
TACACAT7700.0091275045.454545569