FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_Gbc_c78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_Gbc_c78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74568
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGGG200.006926691652.54
GGTCCCA200.006926691652.532
ACACAGG200.006926691652.58
GGAGGTG200.006926691652.555
TAACCAG305.843902E-446.6666645
ATAGTAA500.00716787527.9999981
CTGAGCA901.905439E-423.33333469
CCTGAGC952.7477604E-422.10526368
TGAGCAT900.005231625419.44444570
TCCTGAG1159.960327E-418.26086867
CTCCTGA1553.9920732E-415.80645270
ACTCCTG1807.008612E-515.55555669
GACTCCT1900.001863726812.89473668
AGACTCC2050.003296324311.9512267
CCACGAG3853.1388154E-610.90909269
CGAGACT2903.168483E-410.86206970
GCCCACG5208.813004E-910.76923170
AGCCCAC5502.1114829E-810.18181869
ACGAGAC3105.864352E-410.1612969
CACGAGA3803.2571414E-510.13157870
Produced by FastQC (version 0.11.5)