FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_Gbc_c76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_Gbc_c76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393985
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGAC200.00694866252.50000422
CCTCCTA501.7084478E-435.01
GCGCACC957.617928E-933.15789435
CGCGCGG851.0619806E-732.94117754
CCGGAGC1001.2558303E-831.49999840
GCTCGCG450.00430505731.11111361
ACGGGCC1103.1743184E-828.63636221
CGGGCCG1103.1743184E-828.63636222
AACGCGC1004.4122135E-727.99999852
GACGCGC500.007204709627.9999988
CCGAACG500.007204709627.9999989
GTACTCT906.1620667E-627.2222231
TCACGGG1207.3840056E-826.2519
CGCACCG1207.3840056E-826.2536
GAGGACC700.001225123625.06
GCCGTCA1401.1859811E-825.025
ACTATGC1701.382432E-1024.7058838
CGGGAAC1151.4936577E-624.34782858
CACGGGC1301.6020567E-724.23076820
CCCTTAG750.001830902623.3333341
Produced by FastQC (version 0.11.5)