FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_Gbc_c75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_Gbc_c75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448466
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1205.721795E-520.4163329
GTATTAG9400.016.3827111
TCCGCAC4500.015.55720764
CTAGGAG2553.9060615E-715.0981023
CCCTATA5450.014.7707022
CCGCACC4750.014.73840865
CTATACT10600.014.528364
CGCACCT4650.014.30259366
TAGGTAG5400.014.2593185
TCTATAC10450.014.0670433
TATACCG6350.013.7795835
TATACGG7400.013.7162725
ACCGCGC1800.001259944413.6111678
TAATACT18300.013.3880334
ACAGCGC3152.9008697E-713.3333878
CTAATAC10600.013.2076013
ATCCTAG4801.4551915E-1113.1247851
CCGCGCA2203.932495E-412.7270659
TATACAG11150.012.5561065
GTCCTAG3103.5237608E-612.4191521
Produced by FastQC (version 0.11.5)