FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_Gbc_c73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_Gbc_c73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013525
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC9550.015.3922363
CCCCTAT5100.015.0976131
TCCTTGC14000.013.7498418
GTATTAG11100.013.5581761
CTACCCC4203.2923708E-1013.3329564
CCTTGCG8450.013.2542859
TCCGCAC4801.4551915E-1113.12724364
CGGGGCT2153.239334E-413.0228884
ACGCGCA3507.3983756E-812.99963539
GACGCGC3254.244157E-712.92271138
TCGGGGG2751.4092153E-512.72691316
GTCGGGG3305.1080497E-712.72691315
CCTTGCA10900.012.522799
TCCTATA10150.012.4134442
CCCTATA7700.012.2723812
CCCTTGC7500.012.1331928
CGGTCGG2902.430276E-512.06882513
TGACGCG3802.1606138E-711.97334737
GTATATA27100.011.8815841
TATACCG8850.011.8640725
Produced by FastQC (version 0.11.5)