FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_Gbc_c72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_Gbc_c72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66468
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAAAG150.00221566670.041
ATATACC200.00692339152.54
TTGCGCT200.00692339152.565
ACACTGG200.00692339152.56
AACAGGG200.00692339152.57
ATATTAG350.00124725140.0000041
TTATACC459.108642E-538.8888854
TATACCA400.00240312235.05
GTATTAT450.004279591631.1111091
TAAAGAG450.004279591631.1111095
TATTAGA450.004279591631.1111092
ATTAGAT500.00716234628.0000023
CACGAGA2051.2238805E-515.36585470
CCACGAG2101.5375143E-515.00000169
GCCCACG2352.6771704E-614.89361667
CCCACGA2202.3861987E-514.31818168
AGCCCAC2454.125932E-614.28571466
ACGAGAC1550.00727876613.54838768
GAGCCCA2902.3471654E-512.06896565
CGAGCCC3501.3066858E-511.070
Produced by FastQC (version 0.11.5)