FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_Gbc_c70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_Gbc_c70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603255
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCT7050.020.8504141
CGCGCAT2551.8189894E-1220.58759340
GACGCGC2603.6379788E-1220.19167938
GCGCACT2850.019.6485111
TCCTTGC11700.019.1447038
CCTTGCG5350.018.971379
CTCCTAA4250.018.9405861
CCCCTAG1702.1214728E-618.5288331
ACGCGCA3051.8189894E-1218.36008339
CGCGCGG1351.4132205E-418.14758154
TGACGCG3101.8189894E-1218.06395337
TCCTAAG4700.017.8717822
ACCCTAG2207.280323E-817.4994541
GCGCATG3053.8198777E-1117.21257841
CCCTAGG1452.4406568E-416.8960252
CCGCACG1452.4406568E-416.89602517
CACTCCG2702.415618E-916.85132614
GTCCTAG2552.1787855E-816.4700761
AGCGCAC3651.8189894E-1216.30086310
TAGGTAG3701.8189894E-1216.080585
Produced by FastQC (version 0.11.5)