FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_Gbc_c67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_Gbc_c67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783796
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT2100.024.99915940
CGCGGGA1905.638867E-1022.10451956
CGCGCGG2001.0713848E-920.99929254
GTATCCT6350.020.3930151
CGTCCGG2302.8376235E-1019.78305232
GACGCGC2757.2759576E-1219.09026738
TCCTTGC11600.019.0090478
CCGTCCG2551.1295924E-917.84353831
GTTCCTA7300.017.2596931
TCCGCAC3450.017.24966464
ACGCGCG2852.6739144E-1017.19240453
ACGCGCA2852.6739144E-1017.19240439
GCGCGGG2451.33022695E-817.14227955
CCTTGCG5950.017.058739
TGACGCG2903.4197E-1016.89598337
AGGGGTG1250.001763174316.7999085
CGCACCT3156.184564E-1116.66984466
ACCCGCG2753.083187E-916.54489719
CCTTCTA7200.016.5272221
CTGGGGG1704.389327E-516.47003440
Produced by FastQC (version 0.11.5)