FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_DGY2151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_DGY2151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046850
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC4200.020.83587865
TCCGCAC4250.019.7671264
CGCACCT4400.019.09324366
TAGGTAG5350.017.0089325
TAGGCTG3451.4551915E-1116.2314875
TAGTCAG4350.016.0923469
TAGACTG4650.015.8060665
CTAGACA6000.015.1662964
GCGCACT3503.0559022E-1015.00036611
GTATTAG10150.014.4824061
CCCGAGG1450.00474234814.48240621
GACGCGC2454.2747633E-614.28536538
CGCGCAT2951.3036151E-714.23694140
CTATGCA8450.014.0831849
CCCGTGC1500.00593636314.0003428
CCTAGAC5300.013.8675863
CTAGACT4851.8189894E-1213.7110054
GTGTATA7500.013.5330031
CTAATAC11200.013.4371733
CCTAATA10700.013.4108882
Produced by FastQC (version 0.11.5)