FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_DGY2150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_DGY2150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109464
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCA3650.017.2603559
ACCGCGC1503.1605893E-416.3334128
ACAGCGC2803.914465E-916.2500768
GTATATA26350.016.2042431
CCGCGCG1350.002947603115.555639
TAGGTAG4300.015.4644575
CCCTTGC5400.014.9074788
TATACAG13650.014.6147615
GTATAGA11000.014.3175721
TATACTG13500.014.258655
GTCCTAG3001.6011109E-713.9994031
CTTACAC6250.013.9994033
CCTTGCG5450.013.4863029
GTAGGAC3405.061338E-813.3817823
CCGCACC4203.274181E-1013.33655765
GCGCTCC1600.00908484613.12817563
CTCCTGG5650.013.0082951
GTATTAG9450.012.9624091
TATACGG7100.012.8163545
GCGCAGC3305.1065217E-712.7267356
Produced by FastQC (version 0.11.5)