FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_DGY2144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_DGY2144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533491
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT5350.017.01166366
CCGCACC5700.015.35296865
TAATACT16650.015.3450114
TCCGCAC5750.015.21946264
GTATAGA10350.013.8644321
CGCGCAT3051.958906E-713.77019440
CTATGCA8450.013.6688819
GACGCGC2851.3719437E-613.50847838
TATACAG13250.013.2072615
CTAATAC11750.013.1060983
CTTACAC7600.012.8944573
TATACTG14700.012.8568635
GTATTAG10950.012.4654841
ACGGACT10950.012.46548470
TACTATG11850.012.4052837
GACGGAC11350.012.33453369
ACTATGC10850.012.2582838
TCCTATA8800.011.9315592
TGTAGAC11150.011.9279933
CCGGTCG2100.00401001111.66641358
Produced by FastQC (version 0.11.5)