FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_DGY2143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_DGY2143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520746
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC3100.021.45386964
CCGCACC3150.021.11333365
GGTAGTC4050.019.8773277
TAGGTAG4250.019.7648355
CGCACCT3500.019.00199966
GTATTAG7650.018.7576771
GCTCGCG2056.6665234E-717.07496661
GTATAGA7300.016.3009411
CTAATAC7550.016.2252733
TAGTCAG4150.016.0242029
TAGACTG2854.945832E-915.9650165
GTAGGAG4450.015.72992270
GTGCGAC2503.117093E-715.40060729
TAGGAGT4800.015.31209834
TATACAG11800.015.1272185
ACTGACC2806.8728696E-815.0005928
ACTATGC7300.014.86368
CCGTGTA1656.521517E-414.8485839
GTATATG11300.014.5571391
TACACAG4900.014.2858095
Produced by FastQC (version 0.11.5)