FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_DGY2135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_DGY2135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2455034
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC4250.021.41652164
CCGCACC4600.019.78765
GTCCTAA5600.017.499251
CGCACCT5300.017.17362266
GGGGGGC2653.5000994E-815.8490258
CTAATAC13150.015.7027513
GTATAGA13450.015.6127151
TATACAG18550.014.9050235
GTATTAG12050.014.8126451
GGGGCTC2901.05772415E-714.48272910
GTATATA34950.014.4199851
GTATATG17200.014.0400971
TCTAGAC5500.013.9994013
TAGGTAG7050.013.4036825
ATCAGCC7650.013.26740654
TATACGG9650.013.0564375
CCTATAC7250.013.0339253
CTTAGGG3254.2438296E-712.9225243
GTGTATA11700.012.8626981
GTGCGCA4103.272362E-912.8043354
Produced by FastQC (version 0.11.5)