FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_DGY2090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_DGY2090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2547712
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGCG4200.018.33750761
CCGCACC5100.016.47433965
CTAATAC12800.016.4054783
GTGCGAC5500.015.91021529
TCCGCAC5600.015.62855864
AGCTCGC5000.015.39927560
GCGCAGC5300.015.187963556
CGCACCT5900.014.83388566
GTATAGA10850.014.5154471
TAGGTAG6100.014.3435875
TGCGCAG6150.014.22697255
TATACAG18500.014.1885215
AGTCCGG3504.9039954E-914.00044110
GGTGCGA5850.013.76165728
ACTATGC11800.013.6444978
GTATTAG11600.013.5769471
CACGCAG6700.013.05908616
GTCTAGA5700.012.894131
TCTATAC12500.012.8793943
CAGCTCG6000.012.83272959
Produced by FastQC (version 0.11.5)