FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_DGY2089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_DGY2089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766279
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC4050.023.33729765
TCCGCAC4100.023.05269664
CGCACCT4800.019.69084466
TAGGTAG5050.017.3262425
CTTAGGG2653.4993718E-815.8486093
TTCCGCA7300.015.82460563
TATACGG7300.015.8214695
CTAATAC9750.015.4354593
ATCAGCC7000.014.99957554
GTATTAG8100.014.6909421
TTATACT13400.014.62645154
ACTATGC10650.014.4600948
CATATAC13950.014.300673
CTATACC7750.013.9996024
TCTATAC9250.013.9996023
TAATACT17850.013.9211744
TCAGCCA7300.013.90371555
ATTATAC13900.013.8485293
TACGGTG5850.013.7606847
CCTAGCG2052.1628644E-413.65892330
Produced by FastQC (version 0.11.5)