FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_DGY2086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_DGY2086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974859
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4450.022.808415
CGCACCT3750.020.53645366
TCCGCAC3950.020.38284364
CCGCACC4050.019.01523465
GTATTAG9600.017.4995571
TAGTCAG5050.017.3267339
GGTAGTC5950.017.0588237
ACATAGG5050.016.63408531
GTATAGC4850.015.8758871
CTAGGGT2051.2602573E-515.3654644
ACTATGC10500.015.08
TATTAGG10000.014.6996272
TTAGGTA9550.013.9263494
TACTATG11450.013.7554597
GTATAGA10000.013.6496531
GCGCGGC1550.00737688613.54838712
CTATGCA8550.013.5087739
GTAGGAC2659.619283E-613.2072123
CTATACT10200.013.0388864
GTAGGAG6450.013.02292570
Produced by FastQC (version 0.11.5)