FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_DGY2084.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_DGY2084.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928994
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAG5650.016.7252731
GTATTAG9000.015.9440741
GTATAGA9450.015.5551931
TAGGTAG4950.015.5551925
CGCACCT3401.9645086E-1015.44401866
TCCGCAC3009.727955E-915.16945864
CCTACAC3009.753421E-915.1663133
GTCCTAA4650.015.0534131
TATACCG5650.014.866915
CCGCCCG2151.9772517E-514.6508229
TAGGACG2901.0575968E-714.4824214
TAATACT16700.014.460744
CCTATAC6900.014.2025663
TATTAGG10850.013.5480722
GTCTTAG5300.013.2072381
GTATAAG9550.013.193411
CTAATAC9050.013.1488643
TACACCG3203.5131052E-713.1246955
ACCGTGC2701.1661694E-512.962668
GTATAAT13050.012.8732621
Produced by FastQC (version 0.11.5)