FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_DGY2076.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_DGY2076.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139505
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTG4450.023.59540232
TCCGCAC2207.2759576E-1222.27947264
CGCACCT2451.8189894E-1221.4350666
CCGCACC2501.8189894E-1221.0063665
TAGTGCG5150.020.38826230
TGCGTAC5150.020.38736551
GCGTCTA5000.020.30614764
CGGCATA5600.019.99903525
GCGTACT5300.019.81036652
GAGCGCA1601.1864431E-619.6874149
AGTGCGT5400.019.4443631
GACGTGA4150.018.55413633
ATGCGTC5700.018.42663262
CCCCCTA950.00721074918.4209711
CACGCAG4050.018.14727216
CTTAGAC2901.8189894E-1118.1033693
CGTCTAT5900.017.80265
CTCGGGA1602.600607E-517.505365
TAGCTCG3600.017.505365
CGACGTG4000.017.49992432
Produced by FastQC (version 0.11.5)