FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_DGY2071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_DGY2071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1654504
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC2600.025.58032464
CGCACCT3100.022.58364966
CCGCACC3000.022.16961565
TAGGTAG3850.019.9996385
ACCGCGC1150.001008395818.2605388
GTATTAG6950.018.129171
ACTATGC7550.017.6155748
CTAATAC7350.016.6663653
TATTAGG8500.015.6467742
GTATATG10850.015.4835891
GTCTAAC4750.015.4734051
GTGTATA8150.015.0304031
CTATGCA6150.014.7964799
CCTAGCG2001.7542829E-414.00017130
TAGGACT2001.7547331E-413.9997464
TACAGCG3758.60382E-1013.9997467
CTGATCG2505.2676533E-613.9997469
TAGTCAG3758.60382E-1013.9997469
CTTAGAC4555.456968E-1213.8459033
CCTACAC3354.164758E-813.5818433
Produced by FastQC (version 0.11.5)