FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_DGY1972.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_DGY1972.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412933
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTCG150.00222335870.0042410
CGGGATG450.00430401931.11299527
GGGTCCT657.947839E-426.9247079
TAATACT3250.021.5371574
TATACGG1807.241397E-921.3875945
TACACAG1601.1833326E-619.6863085
CAGCGCA900.00526940619.4456219
TAGGTAG950.0072054918.4199375
GTCTATG950.0072054918.4199371
TATAAGG1902.997367E-718.4199372
ACACTGG1150.001006938518.2597646
CGCCTCT1000.00967679617.5010628
TACACTG1300.002288383416.1528685
CCGAGCC1554.0491662E-415.80549470
ACTATGC1807.129435E-515.5564988
GTATTAG1807.136816E-515.5546141
GTTATAA2503.1045965E-715.3990671
TATATAC5700.015.3499483
ACACAGT1605.154544E-415.3115736
TATAGTG1859.0073714E-515.1360527
Produced by FastQC (version 0.11.5)