FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-H11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-H11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258454
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC2700.023.33639564
TCCGCGG750.00183342423.33268441
CGCACCT2750.022.91209466
CCGCACC2900.021.72698865
GCACGGA1257.828786E-519.59945517
TCCGTCG1401.865685E-417.5002088
CTAATAC6700.017.2383273
ACTATGC6850.016.350568
GACCGAG1450.004739858714.48293112
CTAGGGC1450.004741109414.4823563
CTATGCA6600.014.3183539
ATCAGCC5000.013.99961154
ACTCCGA1750.00101825413.9996116
TATTAGG7100.013.8024332
TAGACAG3303.4169716E-813.7874965
CGACGTG2052.1616596E-413.65924332
GTTACGG2052.1631163E-413.6581572
TTCCGCA4901.8189894E-1213.57320963
GTCTAGA2851.3704648E-613.5083961
GTATATA19500.013.46116351
Produced by FastQC (version 0.11.5)