FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-H09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-H09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495596
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC2050.025.61343864
CCGCACC2259.094947E-1221.78090965
CGACGGG1055.468479E-419.99953349
CCCGGGT950.007212567618.4206213
GTATATA20250.016.765041
CGCACCT2954.3655746E-1016.61255866
CTAATAC7050.016.3825933
GTATTAG6450.016.2786881
TAGGCCG1300.002291589316.1534744
TGCCCCG1400.003755209514.99964910
GTGCGTG3755.4569682E-1114.93398432
ACTATGC6500.014.5381218
ACCGTGT1708.1785873E-414.4114278
GCGCTCG1951.4142756E-414.35959833
AGGCCCG1500.0059372613.99967210
CCCGCCT1500.0059372613.9996728
GTCCTAA3801.0495569E-913.8154661
GTATAGA6700.013.5817711
TAGTGCG4404.7293724E-1113.52331530
TAATACT14350.013.4143214
Produced by FastQC (version 0.11.5)