FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-H07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-H07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009028
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC3550.018.73477265
TAGGTAG4300.017.9070225
CGCACCT3600.017.5022266
TCCGCAC3750.016.80213464
CGTGCGC1250.001763477316.79962310
ACTATGC8450.016.1538878
CCGTGCC1755.6252924E-515.9996419
GCGCGTC2251.7648817E-615.55520836
GTATTAG9100.014.9996651
CTATGCA7900.014.1768989
TACTATG11150.013.8116937
ATGCGCG2801.1237462E-613.74969334
TATACTG13800.013.6956865
TACACCG3354.165304E-813.5821235
CATACAC5700.013.5088063
TAGATAG7300.013.4246915
GTATAGA9450.013.3330351
GTCTATA7350.013.33303451
CTAATAC10350.013.1884383
GCGCACT2405.5686978E-513.12470511
Produced by FastQC (version 0.11.5)