FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-H05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-H05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407497
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA6900.020.7962151
TCTATAC7500.018.1992243
TCCGCAC2005.139518E-717.5042364
CGACCTC1854.753223E-617.026351
CTAATAC7200.017.0131653
ACGGTGT4550.016.9229568
TATACAG11800.016.609465
GCTCGCG2351.5392834E-716.38693861
GTAGGCG1300.002291793716.1531583
CCCGCGC1300.002291793716.15315835
CACGCAG2653.4980985E-815.84838116
TACGGTG4700.015.6381877
GCGCAGC2503.1195123E-715.39934356
ATACGGT5700.015.3502236
TATACGG6400.014.7649955
CGCACCT2656.015307E-714.53181266
CCGGCCC1450.00474082814.48265613
CGCTCCT2202.4558813E-514.31757226
GTGCGAC2454.268566E-614.28662829
GTATATA19250.014.1812131
Produced by FastQC (version 0.11.5)