FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762769
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC2851.2732926E-1118.42293464
TACACAG5000.015.3998255
CCGCACC3009.735231E-915.16821665
GTCCTAA3755.4569682E-1114.9331651
TCGGGGC1450.00474159114.48259458
TAGGTAG3901.0186341E-1014.3588115
GTATTAG7650.014.1828441
CGCACCT3252.7939677E-814.001430566
GTATAGA8250.013.9998411
TATACGG7300.013.9039525
CAGTCCG2303.732786E-513.69549759
GCGCTCG1800.001259846113.61095733
TATACTG12900.013.5657375
TAGACAG4951.8189894E-1213.4341915
TCTAAGC3951.873559E-913.2909883
CGCTCGT1850.001548925913.24309334
ACGGTGT6100.013.1965718
ACTGTCC5051.8189894E-1213.1681678
TATTAGG8550.013.0992662
CATACAC5350.013.0839633
Produced by FastQC (version 0.11.5)