FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-H02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-H02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396075
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG2903.4197E-1016.8960695
ACCGTGC1250.001762765316.800128
TATACCG4400.016.7040675
GTATTAG7150.016.642881
CCTAGCG1704.384E-516.47188830
CGCACCT2452.4758083E-715.71608766
GTATAGA7650.015.555111
TATACTG9700.015.1542055
CCGCACC2553.897676E-715.09977165
ACCGACC1656.5210165E-414.8485918
TAGACAG4251.8189894E-1214.8231045
ACTGTGC2604.862195E-714.8077998
TATATAC20150.014.7638453
GTATAAG6250.014.5595821
ACAGTGT5550.014.5046098
ACTCGCT1450.0047414814.48234415
CTATAGT6300.014.4440314
ACAGCGC1708.1762095E-414.4118688
ATCGCGG1951.4150358E-414.35856251
ACTAGAC4151.8189894E-1114.3369393
Produced by FastQC (version 0.11.5)