FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n01_AP3-G05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n01_AP3-G05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2084376
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC9050.017.402733
TAGACAG5950.016.4700345
GCCCTAG1503.161491E-416.3327851
GTATAGG8250.015.6964431
GTATTAG8500.015.2347821
CCGACGC1400.003755524814.99985713
TATACGG7900.014.176745
GTGCGTG2759.1661786E-714.00053832
CACCGCG2505.2661508E-614.00053728
GTATATA24250.013.5665551
GGCGTCG3102.385841E-713.54890732
GGCTGCG1550.00737753313.5482587
TATACCG6000.013.4162165
GTAGGAC2901.6681206E-613.2754163
CGGGCCT1600.00910133513.12455940
CTACACC6950.013.0930854
CCTACAC2952.0212392E-613.0504083
GTAGACT5103.6379788E-1213.0387774
TAGGTAG4103.270543E-912.8044485
CAGCGTG2751.4084519E-512.7271519
Produced by FastQC (version 0.11.5)